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Image Search Results
Journal: Wellcome Open Research
Article Title: Transient up-regulation of miR-155-3p by lipopolysaccharide in primary human monocyte-derived macrophages results in RISC incorporation but does not alter TNF expression
doi: 10.12688/wellcomeopenres.15065.2
Figure Lengend Snippet: ( A , B ) Human 1°MDMs were exposed to a range of TLR ligands or cytokines for different time periods and total RNA was extracted and cell culture supernatants were collected. Cytokines were IL-1β and TNF, both at 10 ng/ml. TLR ligands were 10 ng/ml LPS (TLR4); 10 ng/ml, poly(:IC) (TLR3); 20 µg/ml, Pam 3 Cys(TLR1/2); 10 ng/ml, MALP-2 (TLR2/6); 30 ng/ml, Flagellin (TLR5); 10 ng/ml and R848 (TLR7/8); 1 µg/ml. ( C , D ) Either primary monocytes or MDMs differentiated with GM-CSF (as opposed to M-CSF) were exposed to LPS for different time periods and total RNA was extracted. ( A , C ) Relative changes to the steady state levels of TNF mRNA and pri-miR-155, miR-155-5p, and miR-155-3p over time; the endogenous control for the miRNAs was miR-16-5p, for TNF and pri-miR-155 it was GAPDH. Mean±SD of technical triplicates. ( B , D ) Cytokines in supernatants were quantified by ELISA. In D the left panel is monocytes, and the right panel macrophages differentiated with GM-CSF.
Article Snippet: Other TLR ligands were: poly(
Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay